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Al-Mehdi, Abu-Bakr, M.D., Ph.D.

Al-Mehdi, Abu-Bakr, M.D., Ph.D.
Assistant Professor
Phone: (251) 460-6704
Fax: (251) 460-6798
aalmehdi@jaguar1.usouthal.edu


Research Interests
  • Mechanotransduction by vascular endothelial cells with flow-adaptation and flow-cessation (ischemia). Shear stress sensors on pulmonary endothelial cells. Endothelial cell reactive oxygen/nitrogen species (ROS/RNS) generation during oxygenated-ischemia in the lung with emphasis on the role of endothelial NADPH oxidase.Role of ROS in cell signaling.
  • Mechanisms of metastasis formation in the lung. Intravascular origin of pulmonary metastasis. Tumor cell-endothelial cell interactions in the pulmonary vasculature.

Research Techniques
  • Intact-organ fluorescence microscopy of subpleural endothelial cells in situ in isolated, perfused rat and mouse lungs. Use of fluorescent probes for plasma- and mitochondrial membrane potential, intracellular ROS/RNS, calcium, free iron, pH, and K+-channels. Cultured bovine pulmonary artery endothelial cells with or without flow-adaptation. Immunohisto- and cytochemistry, spectrofluoro- and photometry

EDUCATION
1987-1990 Ph.D. (Medicine)
Crimea Medical Institute, Simferopol, Crimea , Ukraine
   
1975-1981 M.D. (with Honors)
Crimea Medical Institute, Simferopol, Crimea , Ukraine

PROFESSIONAL EXPERIENCE
2002-present Assistant Professor of Pharmacology,
University of South Alabama, Mobile, AL
   
2000-2001 Research Assistant Professor of Physiology
University of Pennsylvania School of Medicine, Philadelphia , PA
   
1996-2000 Research Associate, Institute for Environmental
University of Pennsylvania, Philadelphia, PA
   
1991- 1996 Postdoctoral Fellow, University ofPennsylvania
School of Medicine, Philadelphia, PA
   
1987-1990 Predoctoral Fellow, Crimea Medical Institute,
Simferopol, Crimea , Ukraine
   
1984-1986 Residency in General Surgery, Crimea Medical
Institute, Simferopol, Crimea, Ukraine
   
1982-1984 Medical Officer, Ministry of Health, Government of
Bangladesh
   
1981-1982 Intern, Rajshahi Medical College Hospital , Rajshahi,
Bangladesh

AWARDS, HONORS
1997 - 2000 Parker B. Francis Fellowship in Pulmonary Research ($108,000).
1993 - 1995 National Research Service Award in Respiratory Physiology, NIH
1984 - 1990 National Scholarship, Ministry of Education, Bangladesh
1974 - 1981 National Scholarship, Ministry of Education, Bangladesh
1970 - 1974 Merit Scholarship, Secondary and Higher Secondary
Education Board, Rajshahi, Bangladesh

MEMBERSHIP
American Physiological Society
American Association for Cancer Research

COURSES TAUGHT DURING THE LAST TWO YEARS
Pharmacology Block VI.

PRINCIPAL INVESTIGATOR OF RESEARCH GRANTS
NIH SBIR
PI of Project: Contrast agents for Far-Red and Near-IR Imaging
10/01-9/03 $67,000
 
NIH CAM SCOR in HBO2 Therapy P50 AT00428-01
Leader of Project 3: Effect of normo- and hyperbaric hyperoxia on the fate of tumor cells and leukocytes in the pulmonary circulation.
9/00-8/05 $600,000
 
Susan G. Komen Breast Cancer Foundation
99-003201: Fate of Breast Tumor Cells in Lung Vessels.
12/99-12/01 $233,990.
 
American Lung Association
RG 5-21337: Role of membrane potential in lung ischemia/reperfusion injury.
7/95-6/97 $50,000.
 
Pennsylvania Lung Association
RG 5-21676: Role of membrane potential in lung ischemia/reperfusion injury.
7/94-6/95 $25,000.

EDITORIAL POSITIONS
Ad hoc Reviewer: Am. J. Resp. Cell Mol. Biol.; Am. J. Resp. Crit. Care Med.
J. Appl. Physiol.;AJP Lung, Free Radical Biol. Med., Circ. Res., Cancer Lett.

PUBLICATIONS
  1. Fu W, Z Zhou, SA Santoro, CM Dipersio, A.B. Al-Mehdi, RJ Muschel. The integrin a3b1 mediates attachment of tumor cells to the pulmonary endothelium. Submitted.
  2. Qiu H, FW Orr, D Jensen, H Wang, AR McIntosh, BB Hasinoff, DM Nance, S Pylypas, C Song, RJ Muschel, A.B. Al-Mehdi. Arrest of B16 melanoma cells in the mouse pulmonary microcirculation induces eNOS-dependent nitric oxide release that is cytotoxic to the tumor cells. Am. J. Pathol. Submitted.
  3. Roy A., Jinqing Li, A.B. Al-Mehdi, Anil Mokashi, and Sukhamay Lahiri. Effect of acute hypoxia on glomus cell Em and øm as measured by fluorescence imaging. J. Appl. Physiol. [In Press].
  4. Wong, C, A. Lee, L. Shientag, J. Yu, Y. Dong, G. Kao, A.B. Al-Mehdi, E.J. Bernhard, R.J. Muschel. Apoptosis: An Early Event in Metastatic Inefficiency. Cancer Res. 2001; 61: 333-338.
  5. Wong, CW., C. Song, MM Grimes, W Fu, MW Dewhirst, RJ Muschel, A.B. Al-Mehdi. Intravascular Location of Breast Cancer Cells after Spontaneous Metastasis to the Lung. Am. J. Pathol. September, 2002.
  6. Wei Z., A.B. Al-Mehdi, A.B. Fisher. Signaling pathway for nitric oxide generation with simulated ischemia in flow-adapted endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 2001; 281: H2226-2232.
  7. Song C., A.B. Al-Mehdi, A.B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am. J. Physiol. Lung Cell. Mol. Physiol. 2001; 281:L993-L1000.
  8. Manevich Y, A.B. Al-Mehdi, V. Muzykantov, A. B. Fisher. Oxidative burst and NO generation as the initial response to "ischemia" in flow-adapted endothelial cells. Am. J. Physiol. Heart Circ Physiol. 2001; 280: H2126-2135.
  9. Wong, C.W., A. Lee, L. Shientag, J. Yu, Y. Dong, G. Kao, A.B. Al-Mehdi, E.J. Bernhard, R.J. Muschel. Apoptosis: An Early Event in Metastatic Inefficiency. Cancer Res. 2001; 61: 333-338.
  10. Al-Mehdi , AB , C. Song, K. Tozawa, A.B. Fisher. Ca2+- and PI3 kinase-dependent nitric oxide generation in lung endothelial cells in situ with ischemia. J. Biol. Chem. 2000; 275:39807-39810.
  11. Haj-Yehia, A., T. Nassar, B.S. Sachais, A. Kuo, K. Bdeir, A.B. Al-Mehdi, A. Mazar, D.B. Cines, A.A. Higazi. Urokinase derived peptides regulate vascular smooth muscle cell contraction in vitro and in vivo. FASEB J. 2000 14: 1411-1422.
  12. Roy, A., C. Rozanov, A. Mokashi, P. Daudu, A.B. Al-Mehdi, H. Shams and S. Lahiri. Mice lacking in gp91phox subunit of NAD(P)H oxidase showed glomus cell [Ca2+]i and respiratory responses to hypoxia. Brain Res. 2000; 872:188-193.
  13. Al-Mehdi, A. B., G. Zhao, K. Tozawa, A.B. Fisher. Depolarization-associated iron release with abrupt reduction in pulmonary endothelial shear stress in situ. Antiox. Redox. Signal. 2000; 2:345-355.
  14. Al-Mehdi, A.B., K. Tozawa, A.B. Fisher, L. Shientag, A. Lee, R.J. Muschel. Intravascularorigin of metastasis from the proliferation of endothelium-attached tumor cells: a new modelfor metastasis. Nature Medicine, 2000; 6:100-102.
  15. Wei, Z., K. Costa, A.B. Al-Mehdi, C. Dodia, V. Muzykantov, A. B. Fisher. Simulated ischemia in flow-adapted endothelial cells leads to generation ofreactive oxygen species and cell signaling. Circ Res 1999 85: 682-689.
  16. Tozawa K.,A. B. Al-Mehdi, V. Muzykantov, A. B. Fisher.In situ imaging of intracellularcalcium with ischemia in lung subpleural microvascular endothelial cells. Antiox. Redox Signal.1999; 1: 145-154.
  17. Al-Mehdi, A.B., A.B. Fisher. Invited Editorial on “Tumor Necrosis factor-a in ischemia and reperfusion injury in rat lungs”. J. Appl. Physiol. 1998; 85: 2003-2004.
  18. Al-Mehdi, A. B., G. Zhao, C. Dodia, K. Tozawa, K. Costa, V. Muzykantov, C. Ross, F. Blecha, M. Dinauer, and A. B. Fisher. Endothelial NADPH oxidase as the source of oxidants in lungs exposed to ischemia or high K+. Circ. Res. 1998; 83: 730-737.
  19. Al-Mehdi, A.B., G. Zhao, and A.B. Fisher. ATP-independent membrane depolarization with ischemia in the oxygen-ventilated isolated rat lung. Am. J. Respir. Cell Mol. Biol. 1997; 18:653-661.
  20. Zhao G., A.B. Al-Mehdi, A.B. Fisher. Anoxia/reoxygenation versus ischemia in isolated rat lungs. Am. J. Physiol. Lung Cell. Mol. Physiol. 1997; 273:L1112-L1117.
  21. Atochina, E.N., V.M. Muzykantov, A.B. Al-Mehdi,S.M. Danilov, A.B. Fisher. Normoxic lung ischemia/reperfusion accelerates shedding of angiotensin converting enzyme from the pulmonary endothelium. Am. J. Respir. Crit. Care Med. 1997; 156:1114-1119.
  22. Al-Mehdi, A.B., H. Shuman, A.B. Fisher. Intracellular generation of reactive oxygen species during non-hypoxic lung ischemia. Am. J. Physiol. Lung Cell. Mol. Physiol. 1997; 272:L294-L300.
  23. Fisher, A.B., A.B. Al-Mehdi. Manifestations and mechanisms of ischemia/reperfusion injury to the lung. In: Oxygen, Gene Expression, and Cellular Function. Clerch, L.B. and Massaro, D.J, eds. Marcel Dekker, Inc., New York , NY , Chapter 14, pp. 309-338, 1997.
  24. Al-Mehdi, A.B., H. Shuman, A.B. Fisher. Oxidant generation with K+-induced depolarization in the isolated perfused lung. Free Rad. Biol. Med. 23:47-56, 1997.
  25. Al-Mehdi, A.B., H. Ischiropoulos, A.B. Fisher. Endothelial cell oxidant generation during K+-induced membrane depolarization. J. Cell. Physiol. 1996; 166:274-280.
  26. Fisher, A.B., A.B. Al-Mehdi. Lipid and protein oxidation in ischemia/reperfusion of the lung. In: Nitric Oxide and Radicals in the Pulmonary Vasculature (ed: Weir, E.K., Archer, S.L., And Reeves, J.T.) Futura Publishing Co, Inc., Armonk , N.Y. 1996; pp 45-61.
  27. Ischiropoulos, H., J. Nelson, D. Duran, A.B. Al-Mehdi. Reactions of nitric oxide and peroxynitrite with organic molecules and ferrihorseradish peroxidase: interference with the determination of hydrogen peroxide. Free Rad. Biol. Med. 1996; 20:373-381.
  28. Ischiropoulos, H., A.B. Al-Mehdi. Peroxynitrite-mediated oxidative protein modifications. FEBS Letters 1995; 364:279-282.
  29. Ischiropoulos, H., A.B. Al-Mehdi, A.B. Fisher. Reactive species in ischemic rat lung injury: Contribution of peroxynitrite. Am. J. Physiol. Lung Cell. Mol. Physiol. 1995; 13:L 158-L 164.
  30. Fisher, A.B., C. Dodia, A.B. Al-Mehdi, H. Ischiropoulos. Ischemia/reperfusion injury in the rat lung: modulation by K+-channel activity. In: The Oxygen Paradox (ed: Davies, K.J.A., and Ursini, F.) Cleup University Press, Padua , Italy . 1995; pp 708-720.
  31. Al-Mehdi, A.B., H. Shuman, and A.B. Fisher. Fluorescence microtopography of oxidative stress in lung ischemia-reperfusion. Lab. Invest., 1994; 70:579-587.
  32. Fisher, A.B., C. Dodia, I. Ayene, A.B. Al-Mehdi. Ischemia/reperfusion injury to the rat lung. Ann. N.Y. Acad. Sci. 1994; 723:197-207.
  33. Al-Mehdi, A.B., C. Dodia, M.Jain,A.B. Fisher. A phospholipase A2 inhibitor decreases generation of thiobarbituric reactive substance during lung ischemia-reperfusion. Biochim. Biophys. Acta 1993; 1167:56-62.
  34. Ayene, S.I., A.B. Al-Mehdi,A.B. Fisher. Inhibition of lung tissue oxidation during ischemia/reperfusion by 2-mercaptopropionylglycine. Arch. Biochem. Biophys. 1993; 303:307-312.
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