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Kane L. Schaphorst, M.D.

Kane L. Schaphorst, M.D.
Associate Professor of Medicine
Phone: (251) 460-7086
Fax: (251) 460-7452
kschaphorst@jaguar1.usouthal.edu

EDUCATION
08/1980 to 05/1984 University of Michigan; Ann Arbor, MI
School of Engineering, B.S.E.
   
08/1984 to 04/1988 University of Nebraska Medical Center; Omaha, NE
School of Medicine, M.D.
   
07/1988 to 06/1989 Indiana University Medical Center; Indianapolis, IN
Intern, Department of Internal Medicine
   
07/1989 to 06/1991 Indiana University Medical Center; Indianapolis, IN
Resident, Department of Internal Medicine
   
07/1991 to 06/1994 Indiana University Medical Center; Indianapolis, IN
Fellow, Division of Pulmonary & Critical Care Medicine

PROFESSIONAL EXPERIENCE
07/1994 to 06/1996 Indiana University Medical Center; Indianapolis, IN
Post-doctoral Fellow, Div. Pulm. & Critical Care Medicine
   
07/1996 to 06/1998 Indiana University Medical Center; Indianapolis, IN
Instructor, Div. of Pulmonary & Critical Care Medicine
   
06/1998 to 11/2003 Johns Hopkins School of Medicine, Baltimore, MD
Asst. Professor, Div. of Pulm. & Critical Care Medicine

RESEARCH ACTIVITIES
Publications
1. Schaphorst KL, Liu F, Jacobs KN, Garcia JGN. Regulation of sphingosine-1-phosphate-induced enhancement of endothelial barrier function: role of F-actin linkage to the zonula adherens via a Rac- and Rho-dependent mechanism. Am. J. Physiol: Lung Molec Physiol 2003 (in press).

2. Schaphorst KL, Chiang E, Jacobs KN, Zaiman A, Natarajan V, Wigley F, and Garcia JG. Role of Sphingosine-1 Phosphate in the Enhancement of Endothelial Barrier Integrity by Platelet-Released Products. Am J Physiol Lung Cell Mol Physiol. 285(1):L258 67, 2003.

3. Young BA, Sui X, Kiser TD, Hyun SW, Wang P, Sakarya S, Angelini DJ, Schaphorst KL, Hasday JD, Cross AS, Romer LH, Passaniti A, Goldblum SE. Protein Tyrosine Phosphatase Activity Regulates Endothelial Cell Cell Interactions, the Paracellular Pathway and Capillary Tube Stability. Am J Physiol Lung Cell Mol Physiol. 285(1):L63 75, 2003.

4. Usatyuk PV, Fomin VP, Shi S, Garcia JGN, Schaphorst KL, Natarajan V. Role of Ca2+ in diperoxovanadate-induced cytoskeletal remodeling and endothelial cell barrier dysfunction. Am J Physiol Lung Cell Mol Physiol. 285(5):L1006 17, 2003.

5. Liu F, Schaphorst KL, Verin AD, Jacobs KN, Birukova A, Day RM, Garcia JGN. Hepatocyte growth factor enhances cytoskeletal rearrangement and endothelial cell barrier and cortical cytoskeletal rearrangement: Potential role of glycogen synthase kinase. FASEB J. 16:950-962, 2002

6. Garcia JGN, Wang P, Liu F, Borbiev T, Becker PM, Schaphorst KL, Verin AD. Critical involvement of p38 MAP kinase in pertussis toxin-induced cytoskeletal reorganization and lung permeability. FASEB J. 16:1064-1076, 2002.

7. Garcia JGN, Schaphorst KL, Patterson CE, Shi S, Verin AD, Natarajan V. Diperoxovanadate alters endothelial cell focal contacts and increases permeability: Role of tyrosine phosphorylation. J. Appl. Physiol. 89:2333-2343, 2000.

8. Patterson CE, Lum H, Schaphorst KL, Verin AD, Garcia JGN. Regulation of endothelial barrier function by the cAMP dependent protein kinase. Endothelium 7:287-308, 2000.

9. Garcia, JGN, Verin AD, Schaphorst KL, Siddiqui R, Patterson CE, Csortos C, and Natarajan, V. Regulation of endothelial cell myosin light chain kinase by Rho, cortactin and p60src. Am. J. Physiol.: Lung Cell. Molec. Physiol., 276:L989 L998, 1999.

10. Shi, S, Verin AD, Gilbert McClain LI, Schaphorst KL, Irwin R, Garcia JGN. Role of tyrosine phosphorylation in thrombin induced endothelial cell contraction and barrier dysfunction. Endothelium, 6 (2): 153 171, 1998.

11. Schaphorst KL, Pavalko FM, Patterson CE, Garcia JGN. Thrombin-mediated focal adhesion plaque reorganization in endothelium. Am. J. Respir. Cell Mol. Biol., 1997; 17:443-455.

12. Garcia JGN, Schaphorst KL, Hart CM, Verin AD, Callahan K, Patterson CE. Mechanisms of ionomycin-induced endothelial permeability. Am. J. Physiol.:Lung Cell. Mol. Physiol., 1997; 273:L172-L184.

13. Garcia JGN, Verin AD, Schaphorst KL. Regulation of thrombin-mediated endothelial cell contraction and permeability. Sem. Thromb. Hemostasis, 1996; 22(4):309-315.

14. Garcia JGN, Schaphorst KL. Regulation of endothelial cell gap formation and permeability. J. Invest. Med. 1994;43:117-126.

15. Patterson CE, Davis HW, Schaphorst KL, Garcia JGN. Mechanisms of cholera toxin prevention of thrombin- and PMA-induced endothelial cell barrier dysfunction. Microvasc. Res. 1994;48:212-235.

16. Patterson CE, Stasek JE, Schaphorst KL, Davis HW, Garcia JGN. Mechanisms of pertussis toxin induced permeability in endothelial cell monolayers. Am. J. Physiol.: Lung Cell. Molec. Physiol. 1994;264:L926-L934.

Extramural Sponsorship (Past)
NIH HL03666, Mentored Clinical Scientist Development Award, Mechanisms of Vascular Barrier Regulation (Schaphorst, KL,). 5 years (1997 2002); $455,153. Principal Investigator (JGN Garcia, Mentor).

EDUCATIONAL ACTIVITIES
CME Instruction
11/1999 “Update in silica related lung disease” in CME course:
Update in Occupational Lung Disease. Eds. J. G. N. Garcia. Baltimore, MD.
   
08/2001 “Update in pleural diseases” in CME course: Update in
Pulmonary Medicine. Eds J.G.N. Garcia, L.S. King, Baltimore, MD.
   
02/2002 “Management of complicated pleural disease” in CME
course: Advanced Pulmonary Diseases. Ed J. Orens, Baltimore, MD

CLINICAL ACTIVITIES
Licenses
09/1998 to 09/2005 Maryland medical license, no. D005383
07/1991 to 09/1999 Indiana medical license, no. 01039804
01/2004 to present Alabama medical license (pending)
 
Certifications
09/1989 National Board of Medical Examiners,
Diplomate.
12/1993 American Board of Internal Medicine
(Internal Medicine), Diplomate.
12/2000 American Board of Internal Medicine
(Pulmonary and Critical Care Medicine), Eligible.

ORGANIZATIONAL ACTIVITIES
Professional Societies
American Thoracic Society, 05/1996 to present
American Society for Cell Biology, 12/1998 to 12/2000.

RECOGNITION
Honors
Tau Beta Pi Engineering Honor Society, 05/1983.
Magna Cum Laude, University of Michigan School of Engineering, 05/1984.
Trainee Investigator Award, AAP/ASCI/AFCR; Baltimore, MD; 05/1992.
Trainee Investigator Award, American Federation for Clinical Research, Midwest Section; Chicago, IL; 11/1992.
Trainee Investigator Award, AAP/ASCI/AFCR; Washington, DC; 05/1993.
Henry Christian Memorial Award for outstanding abstract in pulmonary/critical care research, American Federation for Clinical Research Foundation; Washington DC; 05/1993.
Trainee Investigator Award, AAP/ASCI/AFCR; San Diego, CA; 05/1995.
 
Invited Seminars
07/1999 “The role of myosin light chain kinase in thrombin-induced vascular permeability” in FASEB Summer Seminar series: Thrombin in vascular biology. Saxton River, VT.

11/1999 “Update in silica related lung disease” in CME course: Update in Occupational Lung Disease. Eds. J. G. N. Garcia. Baltimore, MD.

OTHER PROFESSIONAL ACCOMPLISHMENTS
Published Abstracts
 1. Patterson CE, Davis HW, Schaphorst KL, Farber AL, Garcia JGN. Trypsin induces barrier dysfunction by endothelial cell activation. Clin. Res. 1993;41:668A.

2. Schaphorst KL, Pavalko FM, Patterson CE, Garcia JGN. The role of vinculin and talin phosphorylation in thrombin-induced endothelial cell barrier dysfunction. Clin. Res. 1993;41:138A.

3. Schaphorst KL, Pavalko FM, Patterson CE, Garcia JGN. The effect of phorbol myristate acetate stimulation of protein kinase C in endothelium on focal adhesion plaque distribution and vinculin and talin phosphorylation. Clin. Res. 992;40:746A.

4. Schaphorst KL, Patterson CE, Pavalko FM, Garcia JGN. Thrombin induces loss of focal adhesion plaque integrity and vinculin phosphorylation in cultured bovine pulmonary artery endothelium. Clin. Res. 1992;39:307A.

5. Schaphorst KL, Pavalko FE, Patterson CE, Siddiqui R, Garcia JGN. Tyrosine phosphorylation modulates tethering properties in endothelium. Am. J. Respir. Crit. Care Med. 1995; 151(4):653A.

6. Schaphorst KL, Patterson CE, Bruce M, Garcia JGN. Modulation of cadherin/catenin function in endothelium by tyrosine phosphorylation. J. Investig. Med. 1995;43:374A.

7. Schaphorst KL, Shi S, Siddiqui R, Garcia JGN. Ionomycin induces rapid tyrosine dephosphorylation of p125fak. Am. J. Respir. Crit. Care Med. 1996; 152:603A.

8. Schaphorst KL, Patterson CE, Garcia JGN. Role of adherens junction/actin association in PMA- and ionomycin-induced permeability in bovine endothelium. Am. J. Respir. Crit. Care Med. 1997; 155:A783

9. Schaphorst KL, Verin AD, Shi S, Patterson CE, and Garcia JGN. Role of serine/threonine (ser/thr) and tyrosine (tyr) phosphatase activities in ionomycin-induced endothelial permeability. Am. J. Respir. Crit. Care Med., 1998, 157:A531.

10. Patterson CE, Schaphorst KL, Verin AD, Garcia JGN. Regulation of endothelial (EC) barrier function by cAMP-dependent protein kinase (PKA). Am. J. Respir. Crit. Care Med. 1998, 157:A531.

11. Schaphorst KL, Verin AD, Patterson CE, Natarajan N, Garcia JGN. Potential role for FAK tyrosine phosphorylation in the enhancement of endothelial cell barrier function. Am. J. Respir. Crit. Care Med., 161:A755, 2000.

12. Schaphorst KL, Jacobs KN, Verin AD, Garcia JGN. Sphingosine 1-phosphate increases F-actin/ adherins junction linkage and enhances barrier protection. Am. J. Respir. Crit. Care Med. 163:A615, 2001.

13. Jacobs KN, Liu F, Natarajan V, Wadgaonkar R, Schaphorst K, Garcia JGN. Am. J. Respir. Crit. Care Med., 163:A616, 2001.

14. Schaphorst KL, Liu F, Jacobs K, Verin AD, Birukova A, Garcia JGN. Hepatocyte growth factor (HGF)-induced endothelial barrier enhancement: Role of glycogens synthase kinase 3-beta (GSK-3β). Am. J. Respir. Crit. Care Med. 165:A412, 2002.

15. Schaphorst KL, Crow MT, Laiko M, Garcia JGN. Hepatocyte growth factory (HGF)-induced enhancement of endothelial barrier function: Role of Met/grb2-associated binder-1 (Gab1) interaction. Am. J. Respir. Crit. Care Med. 167:A566, 2003
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