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Wu, Songwei, M.D.

Wu, Songwei, M.D.
Assistant Professor
Phone: (251) 460-6366
Fax: (251) 460-6798
swu@jaguar1.usouthal.edu


EDUCATION

1998 - 2001

Postdoctoral Fellow, University of South Alabama College of Medicine, Department of Pharmacology, Mobile, Alabama

1992

M.S., Henan Medical University, Zhengzhou, Henan, China

1986

M.D., Henan Medical University, Zhengzhou, Henan, China

RESEARCH AND PROFESSIONAL EXPERIENCE
2004 – present Assistant Professor
  Center for Lung Biology, University of South Alabama College of Medicine, Mobile, Alabama
   
2002 – present Director
Patch-clamp Electrophysiology Core, Center for Lung Biology, University of South Alabama College of Medicine, Mobile, Alabama
   
2001 – 2004 Instructor
Department of Pharmacology, University of South Alabama College of Medicine, Mobile, Alabama
   
1992 – 1997 Doctor in charge and lecturer
Department of Respiratory Medicine, the 2nd Teaching Hospital of Henan Medical University, Zhengzhou, Henan, China
   
1986 - 1989 Resident Physician
Department of Internal Medicine, the 2nd Teaching Hospital of Henan Medical University, Zhengzhou, Henan, China

HONORS

  • American Heart Association Postdoctoral Fellowship Award, 1999-2001

  • Member, American Heart Association, Lung, Respiration and Resuscitation Review Committee, 2005-2006

MEMBERSHIP

  • American Association for the Advancement of Science, since 12/2003

  • American Thoracic Society, since 1/2003

  • American Physiological Society, since 7/2001

SELECTED PUBLICATIONS
  1. Zhou C, Chen H, King JA, Sellak H, Kuebler WM, Yin J, Townsley MI, Shin HS, and Wu S. The α1G T-type calcium channel selectively regulates P-selectin surface expression in pulmonary capillary endothelium. Am J Physiol Lung Cell Mol Physiol. under review.
  2. Pastukh VM, Chen H, Wu S, Alexeyev MF, and Schaffer SW. Effect of hypernatremia on hypoxic injury: role of T-type Ca2+ channel. Am J Physiol Cell Physiol. 2009 under revision for resubmission.
  3. Wu S, Jian MY, Xu YC, Zhou C, Al-Mehdi AB, Liedtke W, Shin HS, Townsley MI. Ca2+ entry via α1G and TRPV4 channels differentially regulates surface expression of P-selectin and barrier integrity in pulmonary capillary endothelium. Am J Physiol Lung Cell Mol Physiol. 2009;297(4):L650–L657.
  4. Li Y, Liu S, Lu F, Zhang T, Chen H, Wu S, and Zhuang H. A role of functional T-type Ca2+ channel in hepatocellular carcinoma cell proliferation. Oncol Rep. 2009;22(5):1229-1235.
  5. Yin J, Hoffmann J, Kaestle SM, Neye N, Wang L, Baeurle J, Liedtke W, Wu S, Kuppe H, Pries AR, Kuebler WM. Negative-feedback loop attenuates hydrostatic lung edema via a cGMP-dependent regulation of transient receptor potential vanilloid 4. Circ Res. 2008;102(8):966-974.
  6. Lu F, Chen H, Zhou C, Liu S, Guo M, Chen P, Zhuang H, Xie D, Wu S. T-type Ca2+ channel expression in human esophageal carcinomas: a functional role in proliferation. Cell Calcium. 2008;43(1):49-58.
  7. Zhou C, Chen H, Lu F, Sellak H, Daigle JA, Alexeyev MF, Xi Y, Ju J, van Mourik JA, Wu S. CaV3.1 (α1G) controls von Willebrand factor secretion in rat pulmonary microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2007;292(4):L833-844.
  8. Wu S, Chen H, Alexeyev MF, King JA, Moore TM, Stevens T, Balczon RD. Microtubule motors regulate ISOC activation necessary to increase endothelial cell permeability. J Biol Chem. 2007;282(48):34801-34808.
  9. Zhou C, Wu S. T-type calcium channels in pulmonary vascular endothelium. Microcirculation. 2006;13(8):645-656.
  10. Wu S, Cioffi EA, Alvarez D, Sayner SL, Chen H, Cioffi DL, King J, Creighton JR, Townsley M, Goodman SR, Stevens T. Essential role of a Ca2+-selective, store-operated current (ISOC) in endothelial cell permeability: determinants of the vascular leak site. Circ Res. 2005;96(8):856-863.
  11. Rodman DM, Reese K, Harral J, Fouty B, Wu S, West J, Hoedt-Miller M, Tada Y, Li KX, Cool C, Fagan K, Cribbs L. Low-voltage-activated (T-type) calcium channels control proliferation of human pulmonary artery myocytes. Circ Res. 2005;96(8):864-872.
  12. Pastukh V, Wu S, Ricci C, Mozaffari M, Schaffer S. Reversal of hyperglycemic preconditioning by angiotensin II: role of calcium transport. Am J Physiol Heart Circ Physiol. 2005;288(4):H1965-1975.
  13. Lu F, Chen H, Zhou C, Wu S. Is there a role for T-type Ca2+ channel in glioma cell proliferation? Cell Calcium. 2005;38(6):593-595; author reply 597.
  14. Cioffi DL, Wu S, Alexeyev M, Goodman SR, Zhu MX, Stevens T. Activation of the endothelial store-operated ISOC Ca2+ channel requires interaction of protein 4.1 with TRPC4. Circ Res. 2005;97(11):1164-1172.
  15. King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, Stevens T. Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. Microvasc Res. 2004;67(2):139-151.
  16. Wu S, Haynes J Jr, Taylor JT, Obiako BO, Stubbs JR, Li M, Stevens T. CaV3.1 (α1G) T-type Ca2+ channels mediate vaso-occlusion of sickled erythrocytes in lung microcirculation. Circ Res. 2003;93(4):346-353.
  17. Cioffi DL, Wu S, Stevens T. On the endothelial cell ISOC. Cell Calcium. 2003;33(5-6):323-336.
  18. Wu S, Sangerman J, Li M, Brough GH, Goodman SR, Stevens T. Essential control of an endothelial cell ISOC by the spectrin membrane skeleton. J Cell Biol. 2001;154(6):1225-1233.
  19. Brough GH, Wu S, Cioffi D, Moore TM, Li M, Dean N, Stevens T. Contribution of endogenously expressed Trp1 to a Ca2+-selective, store-operated Ca2+ entry pathway. Faseb J. 2001;15(10):1727-1738.
  20. Zhuang H, Bhattacharjee A, Hu F, Zhang M, Goswami T, Wang L, Wu S, Berggren PO, Li M. Cloning of a T-type Ca2+ channel isoform in insulin-secreting cells. Diabetes. 2000;49(1):59-64.
  21. Wu S, Zhang M, Vest PA, Bhattacharjee A, Liu L, Li M. A mibefradil metabolite is a potent intracellular blocker of L-type Ca2+ currents in pancreatic β-cells. J Pharmacol Exp Ther. 2000;292(3):939-943.
  22. Wu S, Moore TM, Brough GH, Whitt SR, Chinkers M, Li M, Stevens T. Cyclic nucleotide-gated channels mediate membrane depolarization following activation of store-operated calcium entry in endothelial cells. J Biol Chem. 2000;275(25):18887-18896.

ABSTRACTS
  1. Townsley MI, Jian MY, Xu YC, Zhou C, Al-Mehdi, AB, Liedtke W, Shin HS, and Wu S.  Ca2+ entry via α1G and TRPV4 channels differentially regulates alveolar capillary endothelium. FASEB J. 2009 23:999.9
  2. Zhou C, Chen H, King JA, Sellak H, Kuebler WM, Yin J, Shin HS, Townsley MI, and Wu S.  A distinct subcellular pool and an α1G T-type Ca2+ channel regulated surface expression of P-selectin in pulmonary capillary endothelium. FASEB J. 2009 23:964.13
  3. King JA, Agarwal S, Syklawer E, Prasain N, Chen H, Resmondo J, McDonald F, Bauer N, Alvarez D, Wu S, Stevens T, Shevde L, Moore T, and Townsley MI. Quantum dots – utilization in TEM. Microsc Microanal. 14(Suppl 2), 2008
  4. Sellak H, Wu S, and Lincoln TM. PKG induces the expression of tumor suppressor genes in colon cancer cells. FASEB J. 2009 23:699.6
  5. Chen H, Wu S, Stevens T, and Cioffi, DL. Calcium phosphate complexation in ISOC channel inactivation. FASEB J, 2008; 22:1178.17
  6. Yin J, Wu S, Neye N, Kuppe H, and Kuebler WM. cGMP regulates mechanosensitive TRPV4 channels in lung endothelial cells. FASEB J, 2008; 22:1213.8
  7. Sellak H, Wu S, and Lincoln TM. Involvement of cGMP-dependent protein kinase in colon cancer cells: analysis of cell proliferation, apoptosis and beta-catenin expression. FASEB J, 2008; 22:819.5
  8. Sellak H, Liu B, Zhou C, Chen H, and Wu S. Mechanism of hypoxia-induced α1H (CaV3.2) gene expression: examining the transcriptional regulation. FASEB J, 2008; 22:960.20.
  9. Pastukh V, Ricci C, Wu S, Gillespie MN, and Schaffer SW. Role of T-type Ca2+ channel in hypernatremic precondition. FASEB J. 2008; 22:751.16
  10. Zhou C, Liu B, Sellak H, Shin HS, and Wu S. α1G T-type Ca2+ channel controls plasma membrane P-selectin expression in pulmonary microvascular endothelial cells. FASEB J, 2007. 21(6): A1433 #977.17.
  11. Wu S, Chen H, King JA, Alexeyev MF, Moore TM, Stevens T, and Balczon RD. Microtubule motors regulate activation of the Ca2+-selective store-operated current, ISOC, important for controlling lung endothelial cell barrier function. FASEB J, 2007. 21(6): A1432 #977.12.
  12. Barry CJ, Cioffi DL, Wu S, Alexeyev MF, and Stevens T. Disruption of the endothelial ISOC causes phenotypic change in pulmonary artery endothelial cells. FASEB J, 2007. 21(6): A1430 #977.2.
  13. Chen H, Lu F, Zhou C, Guo M, and Wu S. T-type Ca2+ channel expression in human esophageal carcinomas: a functional role in proliferation. FASEB J, 2007. 21(6): A538 #604.13.
  14. Kuebler WM, Chen H, Yin J, Zhou C, and Wu S. Depolarization evokes calcium entry trough CaV3.1 channels in lung microvascular endothelium in situ. FASEB J, 2006. 20(4): A749 #469.26.
  15. Chen H, Zhou C, Creighton J, Sellak H, and Wu S. Regulation of CaV3.1 T-type Ca2+ channel expression by cAMP in pulmonary microvascular endothelial cells. FASEB J, 2006. 20(4): A749 #469.25.
  16. Barry CJ, Cioffi DL, Wu S, and Stevens T. Disruption of the proline rich region/protein 4.1 binding domain on the endothelial cell ISOC channel inhibits intercellular gap formation. FASEB J, 2006. 20(4): A748 #469.22.
  17. Prasain N, Wu S, Goodman SR, and Stevens T. Disruption of spectrin-f-actin binding is sufficient to induce intercellular endothelial gaps. FASEB J, 2006. 20(4): A748 #469.21.
  18. Zhou C, Chen H, Daigle JA, van Mourik JA, and Wu S. CaV3.1 (α1G) controls von Willebrand factor secretion in pulmonary microvascular endothelial cells. FASEB J, 2006. 20(4): A748 #469.19.
  19. Rodman DM, Harral J, Wu S, West J, Hoedt-Miller M, Reese KA, and Fagan K. The low-voltage-activated calcium channel CaV3.1 controls proliferation of human pulmonary artery myocytes. Chest. 2005. 128(6 Suppl): 581S-582S.
  20. Pastukh V, Wu S, and Schaffer SW. Role of T-type Ca2+ channel in sodium mediated preconditioning. FASEB J, 2005. 19(5): A1212 #683.3.
  21. Cioffi DL, Alexeyev MF, Wu S, Zhu MX, Goodman SR, and Stevens T.  The essential ISOC channel is gated by interaction of protein 4.1 with TRPC4. FASEB J, 2005. 19(5): A1273 #694.2.
  22. Chen H, Lu F, Zhu B, van Mourik JA, and Wu S. CaV3.1 T-type Ca2+ channel mediates weibel-Palade body exocytosis in lung endothelial cells. FASEB J, 2005. 19(5): A1273 #694.1.
  23. Cioffi DL, Alexeyev MF, Wu S, Zhu MX, Goodman SR, and Stevens, T.  Activation of endothelial ISOC requires interaction between TRPC4 and protein 4.1. FASEB J, 2004. 18(4): A715 #467.5.
  24. Wu S, King JA, Sayner SL, Stevens T, and Balczon RD. Disruption of the dynein motor promotes endoplasmic reticulum-plasmalemma coupling important for activation of the Ca2+-selective store-operated current in pulmonary microvascular endothelium. FASEB J, 2004. 18(4): A715 #467.4.
  25. Cioffi EA, Wallace B, Cioffi DL, Wu S, and Stevens T. Molecular modeling of Fura-2-Be2+ complexes reveals a distorted π aromatic network that modulates its fluorescence detection: implications for use in living endothelia. FASEB J, 2003. 17(4): A414 #309.16.
  26. King JA, Wu S, Cioffi DL, Sayner SL, and Stevens T. Inhibition of type 4 phosphodiesterase induces abluminal translocation of endoplasmic reticulum tubules in lung microvascular endothelial cells. FASEB J, 2003. 17(4): A413 #309.13.
  27. Wu S, King JA, Stevens T, and Balczon RD. Close coupling of endoplasmic reticulum to the plasma membrane is required for ISOC activation in lung microvascular endothelial cells. FASEB J, 2003. 17(4): A411 #309.3.
  28. Wu S, Haynes J Jr, Obiako B, Li M, and Stevens T. T-type Ca2+ channel activation contributes to vaso-occlusion of sickled erythrocytes in inflamed lung. FASEB J, 2002. 16(4): A407 #365.21.
  29. Norwood N, Cioffi DL, Wu S, Dudek S, Garcia J, and Stevens T. Ca2+ store depletion promotes tyrosine phosphorylation of myosin light chain kinase. FASEB J, 2002. 16(4): A467 #365.20.
  30. Wu S, Creighton J, King AJ, and Stevens T. Selective disruption of endothelial cell adhesion by activation of a Ca2+ selective store-operated current, ISOC. FASEB J, 2002. 16(4): A406 #365.16.
  31. Stevens T, Cooper D, Goodman SR, Li M, Dean N, Wu S, Creighton J, Brough GH, Norwood N, and Cioffi DL. Calcium regulation of cAMP production, the control of macro- and micro-vascular endothelial cell function. Journal of Physiology, 2001, 531.P (King's College London - Scientific Meeting): pp5S.
  32. Taylor JT, Wu S, Brough G, Li M, and Stevens T. Selective endothelial expression of T-type voltage-gated Ca2+ channels in lung microvascular cells. FASEB J, 2001. 15(4): A492 #418.7
  33. Cioffi EA, Wu S, Wallace B, and Stevens T. Modulation of Fura-2 luminescence by alkaline earth cations both in vitro and in vivo in pulmonary macro- and microvascular endothelial cells. FASEB J, 2001. 15(4): A492 #418.6.
  34. Norwood N, Wu S, Dudek S, Garcia J, and Stevens T. Endothelial cell myosin light chain kinase-1 and activation of store operated Ca2+ entry. FASEB J, 2001. 15(4): A492 #418.5.
  35. Wu S, Brough GH, Dean N, and Stevens T. Contribution of endogenously expressed trp1 to a Ca2+ selective store operated calcium entry pathway in endothelium. FASEB J, 2001. 15(4): A161 #167.7.
  36. Wu S, Cioffi EA, and Stevens T. Distinct store operated calcium entry pathway in pulmonary macro- and microvascular endothelial cells. FASEB J, 2001. 15(4): A161 #167.6.
  37. Brough GH, Moore TM, Wu S, Dean D, Dean N, and Stevens T. Endogenously expressed trp1 mediates store operated calcium entry in lung cells. FASEB J, 2000, 14(4): A694 #484.15.
  38. Wu S, Brough GH, Creighton J, El-Menshawi M, Goodman SR, and Stevens T. Disruption of F-actin binding to non-erythroid spectrin induces focal inter-endothelial cell gaps. FASEB J, 2000, 14(4): A694 #484.14.
  39. Wu S, Goodman SR, and Stevens T. Non-erythroid spectrin physically links calcium store depletion to activation of calcium entry in endothelial cells. FASEB J, 2000, 14(4): A410 #306.13.
  40. Wu S, Zhang M, Vest PA, Bhattacharjee A, Liu L, and Li M. A mibefradil metabolite is a potent intracellular blocker of L-type Ca2+ currents in pancreatic beta–cells. FASEB J, 2000, 14(4): A110 #108.21.
  41. Wu S, Moore TM, Brough GH, Li M, and Stevens T. Activation of store operated Ca2+ entry causes lung endothelial cell membrane depolarization via a Ca2+-inhibited, cAMP-activated K+ conducting channel. FASEB J, 1999, 13(4): A502 #410.13.

REVIEWS

  1. Zhou C and Wu S. T-type calcium channels in pulmonary endothelium. Microcirculation, 2006, 13(8):645-656.

  2. Cioffi DL, Wu S, and Stevens T. On the endothelial cell ISOC. Cell Calcium, 2003, 33(5-6):323-336.

  3. Wu S. Review of Article “Impairment of store-operated Ca2+ entry in TRPC-/- mice interferes with increase in lung microvascular permeability.” American Thoracic Society – Assemblies: Pulmonary Circulation Journal Club, September 2002, URL: http://www.thoracic.org/assemblies/pc/journal/sept02.asp

BOOK CHAPTERS

  1. Donna L. Cioffi, Songwei Wu, and Troy Stevens. Lung endothelial Phenotypes. Insight derived from the systematic study of calcium channels. In The Pulmonary Endothelium: Function in Health and Disease. Norbert F. Voekel and Sharon Rounds (Ed.). 2009, John Wiley & Sons, Inc. Hoboken, New Jersey, 129-142.

  2. Songwei Wu, Donna L. Cioffi and Troy Stevens. Receptor-operated Ca2+ channels (Chapter 5). In Ion Channels In The Pulmonary Vasculature (Lung Biology in Health and Disease). Jason X-J. Yuan (Ed.). 2005, Taylor & Francis Group. Boca Raton, Florida, 99-124.

  3. Donna L. Cioffi, Songwei Wu, and Troy Stevens. Transient receptor potential cation channels and store operated Ca2+ channels (Chapter 5). In Ion Channels In The Pulmonary Vasculature (Lung Biology in Health and Disease). Jason X-J. Yuan (Ed.). 2005, Taylor & Francis Group. Boca Raton, Florida, 125-146.

PRESENTATIONS

  1. Calcium signals that mediate adhesion of circulating cells. Grover Conference on the Pulmonary Circulation: Genetic and Environmental Determinants of Pulmonary Endothelial Cell Function, Sedalia, CO, September 10, 2004.

  2. Disruption of the dynein motor promotes endoplasmic reticulum-plasmalemma coupling important for activation of the Ca2+-selective store-operated current in pulmonary microvascular endothelium. In Featured Topic Session: Hot Topic in Lung Endothelial Biology, Experimental Biology 2004, Washington, DC, April 21, 2004.

  3. Selective disruption of endothelial cell adhesion by activation of a Ca2+ selective store-operated current, ISOC. In Featured Topic Session: Second Messengers in Lung Cell Function, Experimental Biology 2002, New Orleans, LA, April 24, 2002.

  4. Activation of store operated Ca2+ entry causes lung endothelial cell membrane depolarization via a Ca2+-inhibited, cAMP-activated K+ conducting channel. Experimental Biology 1999, Washington, DC, April 19, 1999.

RESEARCH SUPPORT

ACTIVE

  1. P01 HL66299
    Lung Endothelial Cell Phenotypes — Project 3: T-type calcium channels and von Willebrand factor release
    Project Leader
    08/10/2006-08/09/2011
    Total direct budget: $889,246

  2. R01 HL60024
    Store-operated Ca2+ entry: lung endothelial permeability
    Co-Investigator
    04/10/1998-06/30/2010
    Total direct budget: $978,250

  3. R01 HL92992-01A1
    TRPV4 initiates ventilator induced lung injury
    Co-Investigator
    07/01/2009-06/30/2011
    Total direct budget: $480,000

COMPLETED

  1. R01 HL74116
    Lung endothelium in vaso-occlusion
    Principal Investigator
    04/01/2004-03/31/2009  (Relinquished on August 9, 2006)
    Total direct budget: $1,277,500

  2. American Heart Association Scientist Development Grant
    Role of T-type calcium channels in vaso-occlusion of sickled erythrocytes in the lung microcirculation
    Principal Investigator
    07/01/2002-06/30/2006 (Relinquished on April 1, 2004)
    Total direct budget: $260,000
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